Overlap of Characteristic Serological Antibodies in Rheumatoid Arthritis and Wheat-Related Disorders
Overlap of Characteristic Serological Antibodies in Rheumatoid Arthritis and Wheat-Related Disorders.” Yang Y, Deshpande P, Krishna K, Ranganathan V, Jayaraman V, Wang T, Bei K, Rajasekaran JJ, Krishnamurthy H. Disease Markers. 2019; Article ID 4089178.
Rheumatoid arthritis (RA) and celiac disease (CD) are members of the autoimmune disease family while they have been shown to share multiple aspects in epidemiology and clinical manifestations. In this study, we observed a significantly greater frequency of RA markers in seropositive subjects with wheat-related disorders as well as wheat protein antibodies in RA subjects compared with the respective seronegative controls.
Insights into Cardiovascular Risk and Nutritional Status in Subjects with Wheat-related Disorders
Insights into Cardiovascular Risk and Nutritional Status in Subjects with Wheat-related Disorders.” Siriwardhane T, Krishna K, Devarajan K, Ranganathan, V, Jayaraman V, Wang T, Bei K, Rajasekaran JJ, Krishnamurthy H. Biomarkers. 2019:1-11.
The objective of the study was to explore the lipid profiles and the nutritional status of subjects with wheat-related disorders to understand the potential threat by wheat on cardiovascular risk and nutritional deficiency. Subjects with both Wheat Zoomer positivity (WZ+) and celiac disease positivity had significantly low levels of high-density lipoproteins (HDL) (279/483(57.8%) and 29/47(61.7%) respectively), but only subjects with WZ + had low levels of apo A1 (44/424(9.5%)), and high levels of Omega 6 fatty acids (53/334(15.9%)). None of the micronutrients tested showed a significant imbalance in WZ + subjects.
High Frequency of Extractable Nuclear Autoantibodies in Wheat-Related Disorders
High Frequency of Extractable Nuclear Autoantibodies in Wheat-Related Disorders.” Yang Y, Krishna K, Deshpande P, Ranganathan V, Jayaraman V, Wang T, Bei K, Krishnamurthy H. Biomarker insights. 2018;13:1177271918782893.
In this study, we evaluated the frequencies and levels of autoantibodies, which are important biomarkers of autoimmunity, in subjects with wheat-related disorders and controls. The detected portion of anti-nuclear antibodies (ANA) in wheat-related disorders was very close to the reported occurrence of ANA positivity (15%) in the healthy population, the prevalence of anti- extractable nuclear antigens (ENA) was reported to be less than 2% in the general population; however, our study found it to be much higher in the celiac disease subjects (29%) and the wheat-sensitive subjects (27%), compared with a smaller proportion of seronegative controls (19%).
A Multiplex Autoantibody Panel for Early Detection of Autoimmune
A Multiplex Autoantibody Panel for Early Detection of Autoimmune Disease Activity.” Yang Y, Krishna K, Ranganathan V, Jayaraman V, Wang T, Bei K, Krishnamurthy H, Rajasekaran JJ. Open Journal of Rheumatology and Autoimmune Diseases. 2018;8(2):43-52.
In diagnosis of systemic autoimmune disorders, a cascade autoantibody testing is usually performed by employing antinuclear antibodies (ANA) test as a first screening test and extractable nuclear antigen (ENA) tests as second level determinations. In this study, we evaluated the clinical significance of a multiplex ANA + ENA panel. The two-year follow-up study showed that anti-ENA antibodies may exist years earlier than ANA. Combining ENA tests with ANA screening may reduce false negatives and improve sensitivity.
Exploring Systemic Autoimmunity in Thyroid Disease Subjects
Exploring Systemic Autoimmunity in Thyroid Disease Subjects.” Siriwardhane T, Krishna K, Ranganathan, V, Jayaraman V, Wang T, Bei K, Rajasekaran JJ, Krishnamurthy H. Journal of Immunology Research. 2018; Article ID 6895146.
Individuals with one autoimmune disease are at risk of developing a second autoimmune disease. In this study, we evaluated thyroid hormones, thyroid-stimulating hormone (TSH), free thyroxine (FT4), thyroid autoantibodies, anti-thyroperoxidase (anti-TPO), and anti-thyroglobulin (Tg) to comprehend the association with systemic autoimmune autoantibodies, anti-nuclear antibodies (ANA), and autoantibodies to extractable nuclear antigens (ENA) in subjects with thyroid-related symptoms. During the 2-year follow-up study, anti-TPO appeared significantly earlier than ANA and anti-ENA in an average of 253 (±139) and 227 (±127) days, respectively.
Expanding Immune Reactivity Against Gliadin and TTG Epitopes Long Precedes Celiac Disease Diagnosis
Expanding Immune Reactivity Against Gliadin and TTG Epitopes Long Precedes Celiac Disease Diag nosis.” Choung RS, Jayaraman V, Marietta EV, Rajasekaran J, Wang T, Bei K, Krishnamurthy HK, Murray JA. Gastroenterology. 2018;154(6):S-119.
The aim of this study was to explore the changes of immune recognition against gliadin peptides and tissue transglutaminase in serum samples of a young adult population subsequently diagnosed with CD. Surprisingly, even 6 years before diagnosis, immune responses against gliadin peptides are already present in a subset of celiac patients. This immune reactivity was further augmented as disease progressed from silent to clinically obvious disease.
Reactivity to Neoepitopes of DGP-TTG Complexes can Predict the Healing Status in Treated Celiac Patients
Reactivity to Neoepitopes of DGP-TTG Complexes can Predict the Healing Status in Treated Celiac Patients” Choung RS, Khaleghi S, Marietta EV, Van Dyke CT, Rajasekaran J, Jayaraman V, Wang T, Bei K, Krishnamurthy HK, Snyder MR, Murray JA. Gastroenterology. 2017;152(5):S70.
The aim of this paper is to identify if the pattern of antibody recognition to native and deamidated gliadin derived peptides and tTG as well as novel combinations of peptides derived from both are predictive of mucosal healing of celiac disease (CD). We created neo-epitopes that could be found in a complex of deamidated gliadin peptides and tTG. The loss of the immune responses to the neo-epitopes showed a high sensitivity (84%) and specificity (95%) with a positive predictive value of 0.94 and a negative predictive value of 0.86 to identify healing status of CD.
Determination of B-Cell Epitopes in Patients with Celiac Disease: Peptide Microarrays
Determination of B-Cell Epitopes in Patients with Celiac Disease: Peptide Microarrays.” Choung RS, Marietta EV, Van Dyke CT, Brantner TL, Rajasekaran J, Pasricha PJ, Wang T, Bei K, Krishna K, Krishnamurthy HK, Snyder MR, Jayaraman V, Murray JA. PLoS ONE. 2016;11(1):e0147777.
A novel microarray method for identifying discontinuous B-cell epitopes in celiac disease (CD) by using a silicon-based peptide array and computational methods is presented. Overlapping 12-mer peptide sequences of all native and deamidated gliadins, which are known to trigger CD, were synthesized in situ on the microarray platform and used to identify peptide epitopes. By combining the most discriminative 3-mer gliadin sequences with randomly interpolated 3- or 6-mer peptide sequences, novel discontinuous epitopes were identified and were tested in a confirmatory cohort of CD patients and controls, yielding 99% sensitivity and 100% specificity.
Utilizing High Throughput Discovery Approach to Identify Optimal Candidate Deamidated Gliadin Peptides for the Identification of Celiac Disease.
Utilizing High Throughput Discovery Approach to Identify Optimal Candidate Deamidated Gliadin Peptides for the Identification of Celiac Disease.” Murray JA, Snyder M, Van Dyke CT, Brantner TL, Jayaraman V, Bei K, Wang T, Krishnamurthy HK, Rajasekaran JJ. Gastroenterology. 2013;144(5):S-253.
The aim of this paper is to identify sequences of the deamidated gliadin family (alpha, beta, gamma, omega gliadin) that are most predictive of celiac disease using a high-density in situ synthesized peptide microarrays in combinatorial analysis. Result suggests that antibody recognition of epitopes of DG in CeD is highly sequence specific and only a few sequences are recognized across the entire spectrum of patients with CeD. Similar sequences are recognized by IgA and IgG but thus far the IgA isotype is much more sensitive than IgG (90% vs 68%).